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Imaging Tutorials
We periodically offer short tutorials (~ 3 hrs) on imaging, image processing and analysis.
Seminar: 3D confocal imaging and data analysis
Date: Wednesday April 16, 2008
Location: MIC conference room, BSBW 119
Topics covered include
- resolution limits for 3D imaging
- Sample preparation strategies, choosing the right mounting medium
- optimizing confocal image acquisition parameters
- data processing: noise removal, correction for z-axis compression/expansion
- deconvolution of confocal data
- Software for volume and surface rendering
- Segmentation, volume measurements
- Tracking
Those who plan to attend and have particular imaging/analysis needs are encouraged to email/call Stan Vitha (vitha@mic.tamu.edu; tel. 845-1607) ahead of time so that the issues/concerns can be adequately addressed.
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Download the tutorial slide show from the tutorial held September 2006: Imaging-Tutorial-2006.pdf
Topics:
- Ethical issues: acceptable image processing operations
- File formats for digital micrographs
- Image size, aspect ratio, bit depth
- Noise
- Multi-dimensional data
- Resolution and its limits. Optical artifacts
- Using software for analysis and processing: ImageJ, VOXX
- Import and export of images
- Contrast enhancement, sharpening and other digital filters
- Measurements: spatial and density calibration
- Colocalization analysis
- Image stacks – time lapse and z-series
- Volume rendering and 3D projections