Olympus FV1000 confocal microscope

For imaging services, training, and questions regarding this instrument, please contact Dr. Stanislav Vitha (Tel. 979-845-1607; vitha@tamu.edu).FV1000 confocal microscope

Main features:

  • Inverted IX81 microscope with motorized XY stage, epifluorescence and DIC (Nomarski) illumination
  • New: Two highly sensitive GaAsP detectors, in addition to three existing detectors  
  • New: Live Cell Imaging - environmental enclosure to maintain temperature, CO2 and humidity
  • Spectral imaging  - two detectors with tunable bandwidth
  • SIM scanner for photoactivation/photobleaching at high temporal resolution with a 405 nm laser independently of the imaging lasers.
  • Imaging lasers: 405nm, 458nm, 488nm, 515nm, 543nm, 633nm
  • Objectives: 10x/0.4,  20x/0.75, 40x/0.95 dry,  20x/0.85 oil, 100x/1.4 oil, 60x/1.2 water immersion, also a 60x/0.7 long working distance objective for imaging through thick windows. 
  • NEW: Photometrics Prime sCMOS camera for imaging in non-confocal mode

 

For recommended fluorescent dyes and information on mounting media, go to fluorochromes.html.

You can download the fluorescence spectra of over 500 dyes to your  iPad, iPhone and iPod touch from Zeiss web site: the LightLab App.

All objectives have been tested for resolution and chromatic aberration. Test results are available on request. The motorized stage allows  assembly of large image mosaics and automated imaging of specimens in multi-well imaging chambers. The universal specimen holder accepts standard 1" x 3" slides, 2" x 3" slides, and Petri dishes up to 60mm diameter. The recessed microtiter plate holder accomodates standard-size (85 x 128mm) glass-bottom multiwell culture plates.

A PC with software for off-line analysis of confocal datasets is available in our computer room. The available software includes the full version of the Olympus confocal software and ImageJ (http://rsb.info.nih.gov/ij/). Additionally, the FEI Amira software for image processing,, analysis and  3D reconstruction is available on a separate PC workstation. 

Acknowledment requirements for the Olympus Confocal microscope

MIC guidelines mandate that the use of the MIC facility and the Olympus Confocal Microscope must be properly acknowledged in any publication (including web pages). You may use the following statement:

“The use of the Microscopy and Imaging Center facility at Texas A&M University is acknowledged. The Olympus FV1000 confocal microscope acquisition was supported by the Office of the Vice President for Research at Texas A&M University.”

User are also required to file a copy of any relevant publication containing the acknowledgment with the MIC administrative office.

Downloads:

Short confocal user guide (~10 pages): Olympus_confocal_user_guide.pdf
Requirements for the confocal check-out test: Checkout_Olympus_confocal.pdf
Confocal User manual (Olympus document, 66 pages): FV10-ASW_QuickStart_E.pdf
Free image viewer (for Windows PCs), including extensive help system: download from Olympus here.   Our serial number is 5L10.

User guide for standard epifluorescence imaging on the Olympus system:  Olympus_IX81_epifluorescence_user_manual.pdf