Zeiss Axiophot

For imaging services, training, and questions regarding this instrument, please contact Dr. Stanislav Vitha (Tel. 979-845-1607; vitha@tamu.edu).

axiophot blue2

 

Light microscope equipped for bright field (both transmitted and reflected), phase contrast, transmitted and reflected polarization and Nomarski differential interference contrast microscopy, as well as epifluorescence. Examples of imaging modes are shown below. Motorized focus via the Prior Optiscan system allows acquisition of stacks of optical sections and subsequent deconvolution. The microscope is equipped with color and monochrome CCD cameras. Image acquisition is controlled via the MetaView software and also by the free Micro-Manager software (http://www.micro-manager.org). 

Optics:
2.5x, 5x, 10x, 20x, and 40x dry objectives
63x/1.40 and 100x/1.3 oil immersion objectives

Fluorescence filter sets: includes the set for GFP with a bandpass emission filter that is very effective in suppressing the background autofluorescence. 

excitationdichroic mirroremission filterfluorochrome
G436 nm LP510 LP520 Alexa 430, Mithramycin
BP546 LP580 LP590 Cy3
BP530-585 LP600 LP615 Texas Red
HQ470/20 LP495 HQ525/50 FITC, GFP
BP365 LP400 BP 420-470 DAPI

Note: All wavelengths are in nanometers (nm); BP = bandpass, LP=Longpass filter

Download the Axiophot User Guide: Zeiss-Axiophot-manual.pdf

Examples of the imaging modes:

Example 1: Live cells of the filamentous fungus Neurospora crassa, expressing Green Fluorescen Protein (GFP) fused to Histone H1. The GFP fusion protein is localized in the nucleus.

DIC (Nomarski)

Nomarski

Phase Contrast

phase contrast

Brightfield

brightfield

 Fluorescence

GFP Fluorescence

 Overlay

overlay DIC + fluorescence

Example 2: Hand-cut cross section through a wooded plant stem.

Brightfield

brightfield 1

Darkfield

darkfield 1

Polarization 

polarization

Multi-color Fluorescence

fluorescence 1