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Zeiss Axiophot

by Stanislav Vitha — last modified 2007-08-27 14:13

axiophot blue2

Light microscope equipped for bright field (both transmitted and reflected), phase contrast, transmitted and reflected polarization and Nomarski differential interference contrast microscopy, as well as epifluorescence. Examples of imaging modes are shown below. Motorized focus via the Prior Optiscan system allows acquisition of stacks of optical sections and subsequent deconvolution. The microscope is equipped with color and monochrome CCD cameras. Image acquisition is controlled via the MetaView software and also by the free Micro-Manager software (http://www.micro-manager.org). For more information on this instrument, call or e-mail Stan Vitha.

Optics:
2.5x, 5x, 10x, 20x, and 40x dry objectives
63x/1.40 and 100x/1.3 oil immersion objectives

Fluorescence filter sets: includes the set for GFP with a bandpass emission filter that is very effective in suppressing the background autofluorescence.

excitation	dichroic mirror	emission filter	fluorochrome
G436 nm	LP510	LP520	Alexa 430, Mithramycin
BP546	LP580	LP590	Cy3
BP530-585	LP600	LP615	Texas Red
HQ470/20	LP495	HQ525/50	FITC, GFP
BP365	LP400	BP 420-470	DAPI

Note: All wavelengths are in nanometers (nm); BP = bandpass, LP=Longpass filter

Download the Axiophot User Guide: Zeiss-Axiophot-manual.pdf

Examples of the imaging modes:

Example 1: Live cells of the filamentous fungus Neurospora crassa, expressing Green Fluorescen Protein (GFP) fused to Histone H1. The GFP fusion protein is localized in the nucleus.

DIC (Nomarski)

Nomarski