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January 2010

Histone-GFP fusion protein in Neurospora crassa hyphae. Specimen courtesy of Dr. Bell-Pedersen (Department of Biology). A z-stack of GFP fluorescence images was acquired with z-step of 0.2 um, using Zeiss Axiophot microscope equipped with Plan Neofluar 100x/1.3 oil immersion objective and a Coolsnap cf camera. The raw image stack was processed with AutoDeblur X software (Media Cybernetics) using 100 iterations of blind deconvolution algorithm.  Maximum intensity projection of the raw and deconvolved stacks is shown. Image data was acquired by Laura Short (Department of Anthropology)  during the Spring 2009 Light Microscopy course offered by MIC (BIOL-608, Theory and Applications of Light Microscopy).  For additional views of the deconvolved dataset, go to Deconvolution page in the Guides and Tutorials section.