What's New

MIC Open House

Come and learn about cryo-TEM, confocal/superresolution microscopy and our new BSL-2 culture/live imaging facility. The program includes graduate student poster presentation - the best three posters will receive a prize.  

Friday, October 5, 2018 in the MIC (ILSB 1143)

PLEASE RSVP HERE:  MIC Open House RSVP 

 PROGRAM:

 Cryo-TEM:

  • 9:00-9:30 AM      Dr. Anindito Sen, MIC
  • 9:30-10:00 AM    Dr. Junjie Zhang, Biochemistry/Biophysics
  • 10:00-11:00 AM  Tour of the Facility (Electron Microscopy)
  • 11:00-12:00 noon Graduate Student Poster Session; Prizes will be award for the best three posters.

 

Optical Microscopy, confocal/superresloution:

  • 1:00-1:30 PM      Dr. Stanislav Vitha, MIC
  • 1:30-2:00 PM      Dr. Robert Fuentes, Nutrition
  • 2:00-3:00 PM      Tour of the Facility (Optical Microscopy)

 

 

EM SAMPLE PREPARATION WORKSHOP

 

High Pressure Freezing with Light and Electrical Stimulation using the EM ICE

  • Tuesday, September 11, 2018  9am-11:30am ILSB 1143 (presentation), 1PM-4pm practical demonstration,  ILSB 1127
  • Wednesday September 12, 2018  9am-3pm, ILSB Rm. 1127 - sample preparation

 Hosted by Dr. Stanislav Vitha   (979-845-1607; vitha@tamu.edu)

High pressure freezing captures the intricate changes in fine structure or cellular dynamics. The EM ICE high pressure freezer with light stimulation or electrical stimulation is the tool for optimal sample preparation .for electron microscopy.

Join us for this event and reserve hands-on time with the EM ICE.

The EM AFS2 freeze substitution apparatus with automatic FSP fluid handler, and the laica EM UC7 ultramicrotome with the FC7 cryo-chamber will be also presented. Attendees have an opportunity to bring their own samples for HPF/FS processing and resin embedding.  The workshop is presented by Leica EM sample preparation specialist Dr. Nancy Rizzo (Nancy.Rizzo@leica-microsystems.com).

For more information and to R.S.V.P., please contact:Michael May, 405-788-0775, michaelm@ncimicro.com

 

NEW CONFOCAL/STED/FLIM Imaging System Coming Soon

In summer 2018, a Leica SP8 confocal microscope for live cell imaging with high-speed, multi-spectral, super-sensitivity, super-resolution, and fluorescence lifetime capability will be delivered.

FALCONFALCON - follow fast molecular interactions via FLIM-FRET (Förster Resonance Energy Transfer). Use biosensors to detect changes in metabolic state and microenvironment. Apply lifetime contrast to separate fluorophores. Acquire fluorescence lifetime data with minimal training.

AOBS and WLL (470 nm-670 nm) white light laser source of the Leica TCS SP8 X perfectly matches the wavelength of any fluorophore in the visible spectrum. Up to eight excitation lines can be used – simultaneously. By tuning both excitation and detection, complete excitation emission spectra can be acquired. With this spectral information, any dye can be optimally excited with minimum cross-excitation and specimen damage. If you are able to perfectly hit the excitation peak of your fluorophore you are able to use less laser power leading to less phototoxicity. In addition, the pulsed White Light Laser (WLL) supplies excitation light for fluorescence lifetime measurements. A pulsed 440 laser is coupled with the system for FRET or FLIM-FRET, and 405 nm laser line is included with the system for UV imaging.

LightGate is a filter-free method to remove unwanted signal from the image data. An adjustable time gate switches off the data collection during the white light laser pulse. Additionally, unwanted fluorescence can be removed by adjusting the time gate to obtain the highest image contrast without any reflection contribution.

LIGHTNINGLightning Super-resolution reveals the most information from your images and resolves nanoscale structures down to 120 nm. Obtain real-time results even for ambitious experimental conditions. Maintain resolution and speed for multi-channel data acquisition.

HyD SMD integrated into the Leica SP detector module for gapless multi-spectral imaging. Superior sensitivity allows decreased light dosage. Ideally suited for high-speed imaging. Quantitative through single photon counting. The system includes 3 hybrid detectors, 2 PMTs, and 1 APD. Leica HyD SMD – the universal detector for FCS, FLIM, FLCS and super-sensitive imaging. APD detector fully integrated for higher sensitivity into the far red to handle new NIR probes.

STED

 

STED 775 tunable and direct nanoscopy in x,y (2D) reveals the smallest details (<40 nm). The 775 STED depletion laser coupled with the resonant scanner offers a gentle super-resolution technique for live cell and deep imaging. STED WHITE objectives offer optimal color correction for the full spectrum. Modular concept based on TCS SP8 allows future upgrades to STED 3D or additional depletion lasers.

 

Tandem Scanner for high speed scanning and less phototoxicity to live cells. 22 mm FOV – the largest of any point scanning system. X2Y three mirror scanner for homogenous illumination. Unique combination of FOV and 8 kHz resonant scanning system with switchable galvanometric mirrors. Scan at frequencies of up to 12 kHz, resulting in about 40 fps (512 x 512 px) or 428 fps (512 x 16 px). Acquire 4D stacks up to 50% faster than comparable systems by combining them with the SuperZ GalvoFlow.

Live Cell Imaging - one click of a button and the LED light beam assisted Leica Adaptive Focus Control (AFC) automatically maintains your focus, in real time. Tokai hit stage top incubator for long term live cell imaging maintains humidity, temperature and CO2. System conditions are continuously monitored and saved with the data.

NAVIGATOR

Navigator Software Module - switch from searching image by image to seeing the full overview of your samples. Like a GPS for your cells, the software module LAS X Navigator ensures that you always have a clear roadmap to data. Create fast overviews of your samples and identify the important details instantly. Then set up high resolution image acquisition automatically using templates for slides, dishes and multiwell plates. Allows for high throughput imaging.