This short course covers the principles of optical microscopy with emphasis on fluorescence microscopy techniques. Intended for those who are just starting with fluorescence imaging as well as those who have experience but feel that they need better understanding of some of the techniques.

Students may submit ONE of their own samples ready for imaging to the hands-on session that are nonbiohazard or BSL-1 only (BSL-2 samples are only for Leica SP8 confocal imaging with prior authorization by the MIC).

Lectures/Presentations:

• Image formation
• Limits of resolution
• Principles of fluorescence
• Microscope objectives and their performance
• Cameras and detectors
• Fluorescence dyes, markers and proteins
• Deconvolution
• Optical sectioning
• Confocal
• Lightsheet
• Multiphoton microscopy
• Superresolution microscopy

Practical, hands-on sessions following the lectures!!

(Date, time, and instrument details found in RSVP form.)