Laser Lines, Objectives, and Filters on Each Microscope
Instrument | Objectives | Laser Lines | Filters |
Olympus FV1000 Confocal | 10x/0.4 20x/0.75 40x/0.95 20x/0.85 oil 60x/1.2 water immersion 20x/0.45 60x/0.7 | 405nm 458nm 488nm 515nm 543nm 633nm | |
Leica SP8 Confocal | 10x/0.40 CS2 20x/0.75 multi-immersion 40x/1.10 water immersion 63x/1.30 glycerol immersion 100x/1.40 oil for STED superresolution | 450-670nm white light laser pulsed 440nm 405nm STED 775 tunable | |
Zeiss Z.1 Lightsheet | 20x water immersion 20x for cleared samples 5x for live or cleared samples | 405nm 488nm 561nm 638nm | |
Leica DM6B | 1.25x/0.04 2.5x/0.07 5x/0.15 10x/0.32 20x/0.55 40x/0.60 63x/1.4 oil immersion | GFP with bandpass emission filter | |
Zeiss Axiophot | 2.5x 5x 10x 20x 40x 63x/1.40 oil immersion 100x/1.3 oil immersion |