Monday,December 16 – Thursday December 19, 2024  

Lectures: 9:00am-12:00pm; Hands-on sessions: 1:00-4:00pm

Quiz: Thursday Morning

Cost: $200 for TAMU; Class limit: 16 students

This short course covers the principles of optical microscopy with emphasis on fluorescence microscopy techniques.  Intended for those who are just starting with fluorescence imaging as well as those who have experience but feel that they need better understanding of some of the techniques

Students may submit ONE of their own samples ready for imaging to the hands-on session that are nonbiohazard or BSL-1 only.

Lectures/Presentations:

• Image formation
• Limits of resolution
• Principles of fluorescence
• Microscope objectives and their performance
• Cameras and detectors
• Fluorescence dyes, markers and proteins
• Deconvolution
• Optical sectioning
• Confocal
• Lightsheet
• Multiphoton microscopy
• Superresolution microscopy

Practical, hands-on/Demonstration sessions

• Upright and Inverted Fluorescence Microscopes
• Autoquant and Leica Lightning Deconvolution
• Leica SP8 Confocal/FLIM/STED Superresolution System
• Zeiss Z1 Lightsheet microscope
• Nikon SD Spinning Disk
• Nikon AXR Point-Scanning Confocal