Fluorescence Microscopy Short Course – December 2024
December 16 - 19, 2024
9:00 am - 4:00 pmLectures: ILSB 1134
hands on/Demos: MIC facility microscope rooms in ILSB as well as BSBW 017 (Nikon confocal systems)
Coming in December! $200 registration fee
Past Events
Demo: Leica EM TIC 3x Ion Beam Milling System
August 27 - 28, 2024
12:00 pm - 5:00 pm, 10:00 am - 3:00 pmJune Fluorescence Microscopy Short Course
June 10 - 13, 2024
This short course covers the principles of optical microscopy with emphasis on fluorescence microscopy techniques. It is intended for those who are just starting with fluorescence imaging as well as those who have experience but feel that they need better understanding of some of the techniques.
Fluorescence Microscopy Short Course
December 13, 2023
9:00 am - 1:00 pmLectures:
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- Image formation
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- Limits of resolution
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- Principles of fluorescence
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- Microscope objectives and their performance
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- Cameras and detectors
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- Fluorescence dyes, markers and proteins
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- Deconvolution
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- Optical sectioning
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- Confocal
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- Lightsheet
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- Multiphoton microscopy
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- Superresolution microscopy
SEM Short Course
October 26, 2023
1:00 pm - 4:30 pmStudents may submit ONE of their own samples in advance, on Monday October 23rd, MIC staff will perform sample preparation so that their sample is ready for the practical session.
Note: Before bringing your sample, you must contact Stanislav Vitha, stanvitha@tamu.edu, to confirm the sample is suitable for SEM.
Lectures:
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- Basic principles of SEM: Interactions of electron beam with the sample, types of signals commonly used in SEM (SE, BSE, CL, X-Ray); SEM Design, vacuum system, electron sources, effects of accelerating voltage on resolution, charging, depth, contrast.
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- Analytical SEM : EDS, EBSD – principles, applications, examples
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- SEM of non-conductive samples, environmental SEM, Variable pressure SEM
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- Cryo-SEM, FIB, 3D SEM imaging
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- SEM in surface characterization, texture/roughness analysis, sterogrammetry
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- Principles of sample preparation for SEM
Practical, hands-on session Tuesday October 31 OR Wednesday, November 1, 9am-12pm
- Mounting and sputter coating of samples
- Basic operation of Tescan Vega and FEI Quanta FE SEM
- EDS Analysis
DEMO: 3D Optical Profilometer, Keyence VR-6000
March 30 - 30, 2023
DEMO: Olympus APX100 fluorescence microscope
March 7 - 16, 2023
This all-in-one “Microscope in a box” system is for fluorescence, brightfield and gradient contrast imaging of fixed or live samples on microscope slides, in Petri dishes and culture plates (up to 384 well) with dry as well as immersion optics. Deconvolution of image stacks provides increased contrast and resolution.
The system is designed for ease of use and allows imaging of the entire slide to create an overview, as well as higher resolution imaging and image stitching in manually or automatically selected regions of interest.
https://www.olympus-lifescience.com/en/solutions-based-systems/apx100/
The microscope will be in the MIC from Tuesday, March 7, until Thursday March 16 (exact end of the demo is TBD)
You are encouraged to bring your samples (if live, BSL-1 samples only) and see the new microscope in action.