Skip Navigation

December 2010

FRAP (Fluorescence Recovery After Photobleaching) analysis of a GFP-tagged transcription factor-like protein in Arabidopsis nucleus. TAIR Stock # CS84731 = line N7, which expresses a GFP fusion to a transcription factor-like protein (Cutler et al.,2000.  PNAS 97(7),3718). Photobleaching using the SIM scanner on the Olympus FV1000 confocal microscope and bi-directional scanning permitted image acquisition at high frame rate, with the first post-bleach image (T=260ms) acquired less than 25 ms after bleaching (Bleach T= 227-238 ms). The region of interest (ROI) for bleaching and intensity measurement is indicated by the circle. Image acquired by Stanislav Vitha during the FRAP/RICS imaging tutorial in the MIC, December 1st, 2010.