Confocal z-stack and comparison of confocal vs STED images of Drosophila gut stem cells expressing chimeric (human extracellular, drosophila intracellular) epidermal growth factor receptor (EGFR) labeled with fluorescently conjugated (Alexa 594) anti-human EGFR antibody (Cetuximab). The STED technique is compatible with drosophila gut tissue and provides a significant resolution improvement over confocal, allowing the nanoscale determination of protein clustering in vivo.
Images were acquired on the new Leica SP8 Confocal, STED, and Falcon (FLIM) system microscope by Dr. Robert Fuentes (Dr. Chapkin’s group, Nutrition and Food Science). The sample was prepared by Dr. Mohamed Mlih (Dr. Karpac’s group, Molecular and Cellular Medicine).