This image is a toxic mirror image RNA recruited NONO protein to the nucleolus. HeLa cells were transfected with 200nM toxic mirror image RNA (Red) for 2 hours and stained with anti-NONO primary antibody and Cy3 labeled secondary antibody (Green). Nucleus was stained with Hoechst (Blue). White arrows indicate the nucleolus colocalization of the mirror image RNA and NONO. The image was taken on the Leica SP8 confocal with 40X water immersion objective courtesy of Chen-Hsu Yu in Jonathan Sczepanski’s lab (Chemistry department).