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Instruments

Leica DM 6B

Pricing

Capabilities:

Upright motorized microscope with fluorescence, phase contrast, DIC, polarization, and image stitching.

Specifications:

Imaging Modes:

  • Transmitted light- Brightfield, Polarized Light, Phase Contrast (with 10x, 20x objectives), DIC (with 40x, 63x objectives)
  • Fluorescence-LED light source with filter sets for DAPI, GFP, Cy3, Cy5, CFP, YFP, Texas Red (mCherry, Alexa Fluor 594, ..)

Objectives:

1.25x/0.04, 2.5x/0.07, 5x/0.15, 10x/0.32, 20x/0.55, 40x/0.60 dry, and 63x/1.4 oil immersion. The 40x/0.6 is a dry objective with long working distance and a correction collar for window (coverglass) thickness adjustment, and allows high quality imaging through up to 2 mm glass window.

Camera:

  • Leica DM4500 5-megapixel color camera
  • Leica DFC9000 GT sCMOS, highly sensitive, low noise camera for weak fluorescence signal

Stage: The microscope scanning stage combined with image stitching allows large samples to be imaged or the entire microscope slide at high resolution.

Software:

FOR IMAGING SERVICES, TRAINING, AND QUESTIONS

Please contact Dr. Stanislav Vitha, stanvitha@tamu.edu.


Frequenty Asked Questions, Tips and Helpful Hints:

  • I would like to get trained. How long does it take? Download: DM6B Training Policy
  • How should I prepare my sample for best resolution and fluorescence signal? See this page.
  • Is there a short user manual or use instructions:?- Download the DM6B User Guide
  • Leica LAS X tips and tutorialsLeica Microsystems YouTube channel – Instructional videos on many aspects of image acquisition in LAS X (Navigator, multi-well plate imaging, autofocus, flat field correction, File management, opening LAS X files in FIJI/ImageJ, ..).

Using the MIC? Please cite us by our Research Resource ID: RRID: SCR_022128