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Instruments

Olympus FV1000 confocal microscope

Pricing

Capabilities:

Confocal microscope capable of optical sectioning and 3D imaging of fluorescently labelled samples. Both live (BL1) or fixed, fluorescently-stained samples are both compatible.

Specifications:

Imaging Modes:

  • Fluorescence: time-lapse, Z-stacks, image stitching
  • Transmitted light: DIC

Objectives:

  • 10x/0.4,  20x/0.75, 40x/0.95 dry objectives
  • 20x/0.85 oil, 100x/1.4 oil immersion objectives
  • 60x/1.2 water immersion objectives
  • 20x/0.45  and a  60x/0.7 long working distance objective for imaging through thick (several mm) layers of the specimen

Lasers:

  • 405nm, 458nm, 488nm, 515nm, 543nm, and 633nm imaging lasers
  • SIM scanner for photoactivation/photobleaching at high temporal resolution with a 405nm laser used independently from the imaging lasers

Detectors:

  • 3 standard detectors (2 of these are capable of spectral imaging)
  • Transmitted light detector
  • Two highly sensitive GaAsP detectors

Stage:

  • Inverted IX81 Microscope with a motorized XY stage. The motorized stage allows assembly of large image mosaics and automated imaging of specimens in multi-well imaging chambers.
  • Specimen holders which accept standard microscope slides, Petri dishes up to 60mm in diameter, and standard-size (85 x 128mm) multiwall culture plates.
  • Live cell imaging in an environmental enclosure that maintains temperature, CO₂, and humidity

Software:

PC with software for off-line analysis of confocal datasets is available in our computer room. Available software includes the full version of the Olympus confocal software and ImageJ http://rsb.info.nih.gov/ij/.

FOR IMAGING SERVICES, TRAINING, AND QUESTIONS

please contact Dr. Stanislav Vitha, stanvitha@tamu.edu


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