Olympus FV1000 confocal microscope
|$50/Hour||TAMU Users & Federal Government Agency Users|
|$40/Hour||Off-Peak & Automated Runs
(TAMU Users & Federal Government Users only)
5:00 p.m. - 8:00 a.m.
Confocal microscope capable of optical sectioning and 3D imaging of fluorescently labeled samples. Live (BL1) or fixed, fluorescently-stained samples can be imaged.
In addition, the inverted IX81 microscope is equipped with a sensitive monochrome sCMOS camera and can be used for regular wide-field fluorescence imaging with the Micro-Manager control software. This will require a separate user training (~2 hours). The short user guide for this mode is here: Using the Olympus IX81 for Epifluorescence Imaging
For information on compatible fluorescent dyes, choice of objectives, mounting media and imaging plates/chambers, see this document: https://microscopy.tamu.edu/fluorophore-mounting-media-and-imaging-chamber-selection-for-the-olympus-fv1000-confocal-microscope/
- Fluorescence: time-lapse, Z-stacks, image stitching
- Transmitted light: DIC
- 10x/0.4, 20x/0.75, 40x/0.95 dry objectives
- 20x/0.85 oil, 100x/1.4 oil immersion objectives
- 60x/1.2 water immersion objectives
- 20x/0.45 and a 60x/0.7 long working distance objective for imaging through thick (several mm) layers of the specimen
- 405nm, 458nm, 488nm, 515nm, 543nm, and 633nm imaging lasers
- SIM scanner for photoactivation/photobleaching at high temporal resolution with a 405nm laser used independently from the imaging lasers
FILTER SETS for visual observation (not for confocal imaging):
Excitation and Emission filters are described by a central wavelength and bandwidth, or LP for “longpass” in beamsplitters. For example, 350/50 denotes 325-375nm.
|name||Chroma Cat. #||Excitation||Beamsplitter||Emission|
|Cyan GFP v2 (CFP)||31044v2||436/20||455dclp||480/40|
|Yellow GFP (YFP)||41028||500/20||515lp||535/30|
- 3 standard detectors (2 of these are capable of spectral imaging)
- Transmitted light detector
- Two highly sensitive GaAsP detectors
- Inverted IX81 Microscope with a motorized XY stage. The motorized stage allows assembly of large image mosaics and automated imaging of specimens in multi-well imaging chambers.
- Specimen holders which accept standard microscope slides, Petri dishes up to 60mm in diameter, and standard-size (85 x 128mm) multiwall culture plates.
- Live cell imaging in an environmental enclosure that maintains temperature, CO₂, and humidity
Full version of the Fluoview software is available for off-line analysis on a PC in our computer room, free of charge.
FOR IMAGING SERVICES, TRAINING, AND QUESTIONS
INSTRUCTIONS, DOWNLOAD LINKS AND HOW-TO DOCUMENTS:
- Training Policy: Olympus_FV1000_Training_Policy.pdf
- Quick User Guide: Olympus_confocal_user_guide.pdf
- Checkout Requirements: Checkout_Olympus_confocal.pdf
- Download Image Viewer (PC only): Free from Olympus here
(our serial number is 5L10)
- Fluorescent Protein Database: https://www.fpbase.org/
- Fluorescence spectra viewers:
- Zeiss Light Lab: https://www.zeiss.com/microscopy/int/products/microscope-software/microscopy-apps.html?vaURL=www.zeiss.com/micro-apps